Design of Experiment Approach Based Formulation Optimization of Berberine Loaded Solid Lipid Nanoparticle for Antihyperlipidemic Activity

Berberine is an isoquinoline alkaloid possesses multitude of biological effects. However, quaternary amine cation berberine causes poor water solubility, resulting in low bioavailability which limits its pharmacological purpose. The aim this study was to prepare and optimize loaded solid lipid nanoparticle evaluate pharmacokinetic antihyperlipidemic activity. nanoparticles were prepared by solvent injection method 32 full factorial design used the effect concentration polyvinyl alcohol (X1) amount (X2) on particle size (Y1) entrapment efficiency (Y2). formulation optimized using desirability function evaluated for physicochemical, morphological, vitro drug release vivo study. In activity also studied high fat diet induced hyperlipidemia model. validated experimental comprise 1 % w/v alcohol, 279 mg (stearic acid) achieve 395 nm with 82.44 efficiency. showed initial burst followed slow continuous release. 4.13 folds improvement relative compared suspension. Furthermore, ameliorate levels total cholesterol (-41 %), TG (-49 lipoprotein cholesterol-C (-80 %) high-density cholesterol(+119 hyperlipidemic control found be better than pure drug. are successful delivery system demonstrating effectiveness controlling due improved berberine.